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The use of the stable isotope 44 Ca in studies of calcium incorporation into dentin
Author(s) -
LUNDGREN T.,
ENGSTRÖM E. U.,
LEVISETTI R.,
LINDE A.,
NORÉN J. G.
Publication year - 1994
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1994.tb03437.x
Subject(s) - radiochemistry , calcium , chemistry , isotope , mineralization (soil science) , dentin , isotopes of calcium , ion , stable isotope ratio , mass spectrometry , odontoblast , yield (engineering) , analytical chemistry (journal) , nuclear chemistry , chromatography , materials science , organic chemistry , quantum mechanics , nitrogen , metallurgy , composite material , physics
SUMMARY The incorporation into rat incisor dentin of two calcium isotopes, the stable 44 Ca and the radioactive 45 Ca, was studied using secondary ion mass spectrometry (SIMS) stepscanning and imaging, and autoradiography, respectively. The results demonstrated a time‐dependent incorporation of the calcium isotopes into the mineral phase of dentin. With the SIMS step‐scanning, detecting 44 Ca, the ion yield was high in the odontoblasts 2 min after intravenous injection. After 10 min a marked increase in signal intensity was found at the dentin mineralization front. This result was consistent with those obtained by 45 Ca autoradiography; a peak of incorporation occurred 10 min after injection of the isotope. Likewise, localization of 44 Ca to the mineralization front could be demonstrated 10 min after injection by SIMS imaging. In images obtained at earlier intervals, no such increase in ion yield could be detected. The results show that the nonradioactive, stable isotope 44 Ca can be used as a marker for biomineralization in a similar way to radioactive 45 Ca.