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Volume‐weighted mean particle volume estimation using different measurement methods
Author(s) -
ARTACHOPÉRULA E.,
ROLDÁNVILLALOBOS R.
Publication year - 1994
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1994.tb03429.x
Subject(s) - ruler , equidistant , volume (thermodynamics) , mathematics , standard deviation , logarithm , statistics , physics , geometry , mathematical analysis , quantum mechanics
SUMMARY The use of the ‘point‐sampled intercepts’ method on histopathological material is evaluated for the main purpose of comparing different methods of intercept length measurements. The volume‐weighted mean nuclear volume—nuclear —of the carcinoma of the ampulla of Vater is calculated using three methods for measuring intercept lengths: a semiautomatic image analyser, an equidistant I o ‐ruler and a logarithmic ‐ruler. Rulers of several classes and lengths are used, and the results of the nuclear estimations are compared. The equidistant I o ‐ruler is more accurate than the logarithmic ‐ruler. With a greater number of ruler classes and with adjustment of ruler length to the greatest nuclear intercept lengths, the systematic deviation from the true value of the nuclear is smaller. The nuclear parameter has great power to differentiate intestinal carcinoma from normal tissue.