Premium
Empirically determined freezing time for quick‐freezing with a liquid‐nitrogen‐cooled copper block
Author(s) -
BAATSEN P. H. W. W.
Publication year - 1993
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1993.tb03395.x
Subject(s) - liquid nitrogen , copper , congelation , block (permutation group theory) , chemistry , nitrogen , thermodynamics , mathematics , physics , geometry , organic chemistry
Summary A method is presented to determine freezing time empirically. The method is based on determining the amount of stretch of a skinned muscle fibre while it is being frozen. Freezing time, as determined with this method, lies in between 0·5 and 1·5 ms.