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Video camera calibration for optical densitometry
Author(s) -
BALDOCK R. A.,
POOLE I.
Publication year - 1993
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1993.tb03392.x
Subject(s) - densitometry , calibration , optics , optical density , camera resectioning , computer vision , intensity (physics) , computer science , filter (signal processing) , artificial intelligence , video camera , physics , quantum mechanics
Summary An efficient technique for calibrating video cameras to record optical density (OD) from microscopic images is described. The method corrects for variation over the field of the brightfield and darkfield intensities, does not assume a linear response of the camera to the incident intensity and requires a single calibration filter.