Premium
Estimation of gold‐labelled macromolecules attached to cell surfaces
Author(s) -
THOMAS N.,
GWYNN I. AP
Publication year - 1993
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1993.tb03351.x
Subject(s) - coating , macromolecule , colloidal gold , materials science , scanning electron microscope , analytical chemistry (journal) , optics , chemistry , crystallography , nanotechnology , composite material , chromatography , nanoparticle , physics , biochemistry
Summary A method for obtaining a semi‐quantitative estimation of the amount of colloidal gold label attached to a cell surface is described. The X‐ray emission, in a scanning electron microscope, from an even metal coating applied by diode sputter coating is used as an internal standard. The emission from the standard is used to correct for errors which would have arisen due to factors such as variable specimen surface topography. Examples of the semiquantitative estimation of 10‐nm gold‐labelled wheat‐germ agglutinin to L929 murine fibroblast cells are given.