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Modelling of three‐dimensional fluorescence images of muscle fibres: an application of the three‐dimensional optical transfer function
Author(s) -
SHEPPARD C. J. R.,
GU M.
Publication year - 1993
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1993.tb03310.x
Subject(s) - confocal , pinhole (optics) , optics , optical transfer function , materials science , confocal microscopy , focus (optics) , microscope , filter (signal processing) , transfer function , contrast (vision) , spatial filter , biomedical engineering , computer science , computer vision , physics , engineering , medicine , electrical engineering
SUMMARY Striated muscle fibres can be modelled by a simple geometry, which has allowed three‐dimensional (3‐D) images in conventional and confocal microscopes to be calculated. This model is useful for comparing different imaging methods and represents a simple example of an application of the 3‐D optical transfer function (OTF) for the system. The rejection of out‐of‐focus blur is demonstrated, and the effects of fibre thickness and confocal pinhole size on image contrast are investigated. The effects of using a simple filter for image enhancement are studied, elucidating the characteristics of the OTF.