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Numerical apertures of light microscope objectives
Author(s) -
LEHMANN W.,
WACHTEL A.
Publication year - 1993
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1993.tb03282.x
Subject(s) - optics , numerical aperture , magnification , microscope , simplicity , lens (geology) , instrumentation (computer programming) , aperture (computer memory) , physics , materials science , computer science , acoustics , wavelength , quantum mechanics , operating system
SUMMARY The numerical aperture of light‐microscope objectives is measured via the exit angle of the rear lens towards the image space, and the magnification of the objective. The method is reliable because of its simplicity and is independent of special instrumentation such as apertometers. Results from eight commercially available objectives indicate fair agreement of nominal data with measured data for the magnifications, but not for all numerical apertures.