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A simple modification to the LKB 7800 series Knifemaker and a balanced‐break method to prepare glass knives for cryosectioning
Author(s) -
WEBSTER P.
Publication year - 1993
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1993.tb03281.x
Subject(s) - simple (philosophy) , series (stratigraphy) , materials science , computer science , biology , paleontology , epistemology , philosophy
SUMMARY A novel method of using the LKB 7800 series Knifemaker to produce glass knives using a balanced break is described. The method produces knives of sufficient quality to section aldehyde‐fixed, sucrose‐cryoprotected, frozen biological material in a cooled cryochamber of an ultramicrotome. The modifications to the Knifemaker are minimal and, if required, the machine can be returned easily to its normal state after use.