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A method for spatio‐temporal (4‐D) data representation in confocal microscopy: application to neuroanatomical plasticity
Author(s) -
KRIETE A.,
WAGNER H.J.
Publication year - 1993
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1993.tb03275.x
Subject(s) - confocal , hue , volume rendering , visualization , computer science , artificial intelligence , computer vision , pattern recognition (psychology) , confocal microscopy , representation (politics) , microscopy , rendering (computer graphics) , data set , optics , microscope , biological system , biology , physics , politics , law , political science
SUMMARY This paper describes a new method for data representation and visualization in four dimensions (three dimensions plus time). Sequential volumes, exhibiting morphological activity, are acquired non‐invasively with a confocal scanning laser microscope, where each data set corresponds to a time sample. A pipelined processing includes packing of volumes and specific volume rendering techniques. Subsequent processing in HIS (hue, intensity, saturation) colour space combines functional, packed images with shaded three‐dimensional views. As a result, even subtle changes in morphology become visible and computational time is saved. Experimental findings obtained from investigations of synaptic plasticity in cultured retinal tissue are reported.

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