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Measuring the surface area of a cell by the method of the spatial grid with a CSLM—a demonstration
Author(s) -
Howard C. Vyvyan,
Sandau Konrad
Publication year - 1992
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1992.tb04315.x
Subject(s) - stack (abstract data type) , grid , surface (topology) , grid cell , confocal , optics , microscope , materials science , biological system , computer science , mathematics , geometry , physics , biology , programming language
SUMMARY The spatial grid is a method for estimating the surface area of particles. A stack of perfectly registered sections is the essential prerequisite for its use. The confocal scanning light microscope provides such a stack by optical sectioning. The spatial grid method is briefly described and applied to an osteocyte lacuna in dry mineralized human mandible. This type of cell was chosen because of its very complex shape. The variance of the area estimate is studied and compared with the results of a simulation.