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Confocal differential interference contrast (DIC) microscopy: including a theoretical analysis of conventional and confocal DIC imaging
Author(s) -
Cogswell C. J.,
Sheppard C. J. R.
Publication year - 1992
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1992.tb04307.x
Subject(s) - differential interference contrast microscopy , confocal , vignetting , confocal microscopy , optics , contrast (vision) , interference microscopy , phase contrast microscopy , interference (communication) , microscope , materials science , microscopy , prism , differential (mechanical device) , lens (geology) , physics , computer science , computer network , channel (broadcasting) , thermodynamics
SUMMARY A technique for obtaining differential interference contrast (DIC) imaging using a confocal microscope system is examined and its features compared to those of existing confocal differential phase contrast (DPC) techniques as well as to conventional Nomarski DIC. A theoretical treatment of DIC imaging is presented, which takes into account the vignetting effect caused by the finite size of the lens pupils. This facilitates the making of quantitative measurements in DIC and allows the user to identify and select the most appropriate system parameters, such as the bias retardation and lateral shear of the Wollaston prism.