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Digital image acquisition in in vivo confocal microscopy
Author(s) -
Petroll W. M.,
Cavanagh H. D.,
Lemp M. A.,
Andrews P. M.,
Jester J. V.
Publication year - 1992
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1992.tb04305.x
Subject(s) - confocal microscopy , confocal , microscopy , in vivo , materials science , confocal laser scanning microscopy , computer vision , chemistry , artificial intelligence , optics , computer science , biophysics , biology , physics , microbiology and biotechnology
SUMMARY A flexible system for the real‐time acquisition of in vivo images has been developed. Images are generated using a tandem scanning confocal microscope interfaced to a low‐light‐level camera. The video signal from the camera is digitized and stored using a Gould image processing system with a real‐time digital disk (RTDD). The RTDD can store up to 3200 512 times 512 pixel images at video rates (30 images s −1 ). Images can be input directly from the camera during the study, or off‐line from a Super VHS video recorder. Once a segment of experimental interest is digitized onto the RTDD, the user can interactively step through the images, average stable sequences, and identify candidates for further processing and analysis. Examples of how this system can be used to study the physiology of various organ systems in vivo are presented.