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Prospects for confocal imaging based on nanosecond fluorescence decay time
Author(s) -
Morgan C. G.,
Mitchell A. C.,
Murray J. G.
Publication year - 1992
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1992.tb04304.x
Subject(s) - confocal , microscope , nanosecond , fluorescence , contrast (vision) , optics , fluorescence lifetime imaging microscopy , detector , fluorescence microscope , confocal microscopy , microscopy , physics , multiphoton fluorescence microscope , materials science , photon , laser
SUMMARY A fluorescence microscope has been developed to give contrast on the basis of fluorescence decay time. Two different detection schemes are used, one based on an imaging single photon detector equipped with an external photon correlator and the other using an RF‐modulated intensified CCD camera. Examples of the new contrast are shown, and the possible routes to development of a confocal version of the microscope are discussed.