Prospects for confocal imaging based on nanosecond fluorescence decay time | Zendy

Morgan C. G. | Zendy; Mitchell A. C. | Zendy; Murray J. G. | Zendy

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Prospects for confocal imaging based on nanosecond fluorescence decay time

Author(s) -

Morgan C. G.,

Mitchell A. C.,

Murray J. G.

Publication year - 1992

Publication title -

journal of microscopy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.569

H-Index - 111

eISSN - 1365-2818

pISSN - 0022-2720

DOI - 10.1111/j.1365-2818.1992.tb04304.x

Subject(s) - confocal , microscope , nanosecond , fluorescence , contrast (vision) , optics , fluorescence lifetime imaging microscopy , detector , fluorescence microscope , confocal microscopy , microscopy , physics , multiphoton fluorescence microscope , materials science , photon , laser

SUMMARY A fluorescence microscope has been developed to give contrast on the basis of fluorescence decay time. Two different detection schemes are used, one based on an imaging single photon detector equipped with an external photon correlator and the other using an RF‐modulated intensified CCD camera. Examples of the new contrast are shown, and the possible routes to development of a confocal version of the microscope are discussed.

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