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Low‐energy electron microscopy (LEEM) and mirror electron microscopy (MEM) of biological specimens: Preliminary results with a novel beam separating system
Author(s) -
Griffith O. Hayes,
Hedberg Karen K.,
Desloge Denis,
Rempfer Gertrude F.
Publication year - 1992
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1992.tb03267.x
Subject(s) - low energy electron microscopy , electron microscope , electron , cathode ray , microscopy , physics , optics , biological specimen , materials science , nuclear physics
SUMMARY Low‐energy electron microscopy (LEEM) and mirror electron microscopy (MEM) utilize a parallel beam of slow‐moving electrons backscattered from the specimen surface to form an image. If the electrons strike the surface an LEEM image is produced and if they are turned back just before reaching the surface an MEM image results. The applications thus far have been in surface physics. In the present study, applications of LEEM and MEM in the biological sciences are discussed. The preliminary results demonstrate the feasibility of forming images of uncoated cultured cells and cellular components using electrons in the threshold region (i.e. 0–10 V). The results also constitute a successful test of a novel beam‐separating system for LEEM and MEM.