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Pattern reconstruction in ultrastructural morphology
Author(s) -
Bellon Pier Luigi,
Lanzavecchia Salvatore
Publication year - 1992
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1992.tb03248.x
Subject(s) - ultrastructure , iterative and incremental development , morphology (biology) , orientation (vector space) , process (computing) , computer science , materials science , biological system , optics , algorithm , computer vision , artificial intelligence , mathematics , physics , geometry , biology , anatomy , operating system , software engineering , genetics
SUMMARY A method of image reconstruction which can be used in ultrastructural morphology when a number of comparable structures are available is described. The method is suitable for quasi‐regular arrays of objects, and an extension of it allows the average of equivalent objects which could be orientated at random to be obtained. When dealing with crystalline arrays it is first necessary to obtain a leakage‐free pattern in which the coarse distortions are suppressed; this pattern is then filtered to obtain a reference. When dealing with several individual objects which must be brought to the same orientation and enlargement, one of the objects is fixed as a reference. In both cases the distortions relative to the reference are eliminated by an iterative process, making a final average possible. In crystalline arrays this method enhances the bandwidth of the pattern in a way that can be estimated quantitatively by figures of merit. Two examples of reconstructions are reported and discussed: a ctenophore macrocilium, regarded as a crystalline structure, and a sectioned flagellum of an insect spermatid.