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An improved cryo‐jet freezing method
Author(s) -
Greene William B.,
Walsh Lyle G.
Publication year - 1992
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1992.tb01519.x
Subject(s) - jet (fluid) , cryo electron microscopy , substitution (logic) , materials science , biophysics , biomedical engineering , chemistry , computer science , thermodynamics , biology , physics , medicine , programming language
SUMMARY A new cryo‐jet freezing apparatus is described that is easy to use and gives good results using a propane‐butene mixture (3: 1). Our use of the freezer in the study of mouse spinal cord explant cultures is discussed. At the tissue surface, the quality of tissue preservation from freezing, followed by freeze substitution, rivals that of conventional electron microscopic methods. Certain intracellular structures are better visualized using our methods. There is no evidence of the tissue being distorted by the cryogen jet when the freezer is operated correctly. A new freeze substitution device is also discussed.