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Molecular histochemistry and plant biology: a review
Author(s) -
Harris N.
Publication year - 1992
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1992.tb01503.x
Subject(s) - biology , homeotic gene , in situ hybridization , ontogeny , computational biology , gene , gene expression , evolutionary biology , microbiology and biotechnology , biochemistry , genetics
SUMMARY This review considers some of the recent advances in the applications of molecular histochemical techniques to the analysis of the temporal and spatial patterns of gene expression associated with differentiation and development in plant tissues. A range of methods is outlined, and those for tissue preparation and the isotopic and non‐isotopic labelling of probes for in situ hybridization studies are discussed, taking account of the various approaches which can be used for the localization of specifically bound probes. An overview of some successful applications of molecular histochemistry includes the elucidation of the roles of homeotic genes in floral biology, the ontogeny and differentiation of the photosynthetic apparatus, and aspects of seed development, including storage protein gene regulation during embryogenesis.