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Effect of refraction on optical microscopic measurement of internal blood‐vessel diameter and its correction
Author(s) -
Chen Eric Q.,
Lam Chan F.,
Periasamy Ammafi
Publication year - 1991
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1991.tb03210.x
Subject(s) - refraction , optics , materials science , physics
SUMMARY In optical microscopic measurement of internal blood‐vessel diameters, the effect of refraction must be taken into account to ensure accuracy of the result. This effect is discussed and an analytical correction formula derived. Phantom blood vessels with known internal and external diameters were used to test the validity of the correction formula. The errors obtained prior to correction were reduced significantly after correction.