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A device to ease grid coating for EM autoradiography
Author(s) -
Riboni Laura,
NúñezDurán Harol
Publication year - 1991
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1991.tb03203.x
Subject(s) - coating , groove (engineering) , grid , materials science , computer science , process (computing) , composite material , mechanical engineering , geology , metallurgy , engineering , operating system , geodesy
SUMMARY A modification to the standard device used to hold grids during the process of coating with emulsion for EM autoradiography is described. The device has a plastic base with six bronze poles attached. Each pole has a groove machined along its side to the top, and a shallow depression at the end of the groove. Instructions for the construction of the poles and for use of the device are given. The innovative design allows the user to feel the top of the pole, and thus simplifies selection of the coated grids. As grids are less exposed to safe light than in the standard procedure, the background of silver grains decreases; also the chance of missing a grid decreases.