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The experimental effect of detector size on confocal lateral resolution
Author(s) -
Glass Monty,
Dabbs Tim
Publication year - 1991
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1991.tb03200.x
Subject(s) - optics , apodization , microscope , lens (geology) , confocal , resolution (logic) , 4pi microscope , detector , materials science , numerical aperture , confocal microscopy , aperture (computer memory) , physics , conventional transmission electron microscope , computer science , scanning electron microscope , artificial intelligence , scanning transmission electron microscopy , wavelength , acoustics
SUMMARY The lateral resolution of a scanning‐spot microscope has been measured as the product of the pin‐hole size and lens numerical aperture of the detector approaches the confocal limit. The resolution follows an earlier prediction, improving approximately 30% in going from that of a conventional scanning‐spot or broad‐viewing‐area microscope to that of a fully confocal microscope. The discrepancy between the data and the theoretical curve can be attributed to lens apodization arising from the use of multi‐element thick lenses and a non‐ideal, truncated Gaussian beam profile.