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Atomic force microscopy of a hydrated bacterial surface protein
Author(s) -
Wiegräbe Winfried,
nenmacher Martin,
Guckenberger Reinhard,
Wolter Olaf
Publication year - 1991
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1991.tb03161.x
Subject(s) - transmission electron microscopy , atomic force microscopy , microscopy , layer (electronics) , conductive atomic force microscopy , materials science , deinococcus radiodurans , electron microscope , scanning tunneling microscope , chemistry , nanotechnology , crystallography , analytical chemistry (journal) , chemical physics , optics , physics , chromatography , biochemistry , gene
SUMMARY The protein surface layer of the bacterium Deinococcus radiodurans (HPI layer) was examined with an atomic force microscope (AFM). The measurements on the air‐dried, but still hydrated layer were performed in the attractive imaging mode in which the forces between tip and sample are much smaller than in AFM in the repulsive mode or in scanning tunnelling microscopy (STM). The results are compared with STM and transmission electron microscopy (TEM) data.