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A device for freeze‐substituting a large number of samples under controlled conditions
Author(s) -
Marshall A. T.,
Kent M.
Publication year - 1991
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1991.tb03144.x
Subject(s) - reciprocating motion , shaker , liquid nitrogen , substitution (logic) , microprocessor , refrigerator car , materials science , chromatography , analytical chemistry (journal) , chemistry , computer hardware , computer science , mechanical engineering , acoustics , engineering , physics , organic chemistry , gas compressor , vibration , programming language
SUMMARY An inexpensive device for reproducible freeze‐substitution is described. The equipment consists of a reciprocating shaker, holding vials of substitution fluid, mounted in the gas phase of a large liquid nitrogen refrigerator. The shaker temperature is controlled by a programable microprocessor.