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A quantitative computer simulation of microscopic imaging
Author(s) -
Goldstein D. J.
Publication year - 1991
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1991.tb03134.x
Subject(s) - optics , apodization , aperture (computer memory) , diffraction , transmittance , contrast (vision) , grating , fourier transform , schlieren , interference (communication) , differential interference contrast microscopy , spherical aberration , phase (matter) , physics , materials science , computer science , microscopy , computer network , channel (broadcasting) , quantum mechanics , acoustics , lens (geology)
SUMMARY A versatile program is described for a BBC‐ or IBM‐compatible microcomputer, which uses a fast Fourier transform in calculating diffraction‐limited microscopic images. The defined ‘object’ can be a diffraction grating or a discrete specimen seen in cross‐section, of arbitrary interval (or width), transmittance and retardation. Many microscopical techniques can be simulated including bright‐field, phase contrast, central and peripheral dark‐field, schlieren, apodization, fluorescence, differential interference contrast and confocal scanning. Instrumental settings which can be varied include the aperture, focus and primary spherical aberration of the objective, the coherence and obliquity of the illumination, and in phase contrast the width, transmittance and retardation of the phase plate. The Fourier transform and various other algorithms used in the program are explained.