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A simplified preparation procedure of plant material for elemental analysis by ESI and EELS techniques
Author(s) -
Lehmann H.,
Kunz U.,
Jacob A.
Publication year - 1991
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1991.tb03117.x
Subject(s) - elemental analysis , sulfur , algae , phosphorus , electron microscope , chemistry , materials science , chemical engineering , nanotechnology , mineralogy , biology , botany , inorganic chemistry , metallurgy , optics , physics , engineering
SUMMARY Living plant cells were embedded in the water‐soluble resin Nanoplast FB 101 and cut into ultrathin sections. Non‐vacuolated cells of algae and fungi showed a satisfactory preservation of fine structure. First results of elemental mapping by the ESI technique indicate that phosphorus and sulphur are neither extracted nor dislocated within the cells. The authors are members of the ‘Study Group for Electron Microscopy’ at the Hannover School of Veterinary Medicine.