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Quantitative analysis of electron energy‐loss spectra from ultrathin‐sectioned biological material
Author(s) -
Sorber C. W. J.,
Ketelaars G. A. M.,
Gelsema E. S.,
Jongkind J. F.,
Bruijn W. C.
Publication year - 1991
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1991.tb03113.x
Subject(s) - electron energy loss spectroscopy , transmission electron microscopy , elemental analysis , electron , ionization , enhanced data rates for gsm evolution , materials science , spectroscopy , biological specimen , spectral line , ionization energy , analytical chemistry (journal) , computational physics , optics , atomic physics , computer science , chemistry , physics , nanotechnology , ion , artificial intelligence , organic chemistry , chromatography , quantum mechanics , astronomy
SUMMARY A computer program for quantitative spectral analysis is proposed for the elemental analysis of biological material by electron energy‐loss spectroscopy in a conventional transmission electron microscope, the Zeiss EM902. Bio‐standards are used to test the performance of this program. The application of a simplex optimization method for curve‐fitting is proposed to separate the ionization edge from the background. Making use of Ce‐, Ca‐ and Fe‐bio‐standards, this method is compared with Egerton's well‐known two‐area method.