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A simple handling technique for mammalian oocytes and embryos during preparation for transmission electron microscopy
Author(s) -
Britton Ann P.,
Moon Young S.,
Yuen Basil Ho
Publication year - 1991
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1991.tb03107.x
Subject(s) - transmission electron microscopy , electron microscope , staining , centrifugation , negative stain , capsule , embryo , microscopy , materials science , computer science , biophysics , chemistry , biomedical engineering , microbiology and biotechnology , biology , nanotechnology , chromatography , optics , physics , genetics , medicine , botany
SUMMARY Due to their small size, mammalian oocytes and embryos pose unique problems during preparation for transmission electron microscopy. This paper outlines a method which combines protein embedding with centrifugation to locate the specimens on the face of a Beem capsule mould. This method facilitates both the processing of oocytes with minimal loss and rapid location of the specimens within the block for simultaneous sectioning, staining and examination.