Premium
Three‐dimensional image formation in confocal microscopy
Author(s) -
Sheppard C. J. R.,
Cogswell C. J.
Publication year - 1990
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1990.tb04774.x
Subject(s) - confocal , microscope , optics , confocal microscopy , transverse plane , microscopy , scanning confocal electron microscopy , materials science , detector , physics , anatomy , biology
SUMMARY Three‐dimensional (3‐D) imaging in confocal microscopes is considered in terms of 3‐D transfer functions. This leads to an explanation of axial imaging properties. The axial response was observed in both object‐scanning and beam‐scanning microscopes and the influence of off‐axis examination investigated. By simple processing of multi‐detector signals, imaging in both the axial and transverse directions can be improved.