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A rapid preparation method for ultrastructural investigations of conifer needles
Author(s) -
Ebel B.,
Hamelmann U.,
Nieß C.
Publication year - 1990
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1990.tb03048.x
Subject(s) - ultrastructure , autolysis (biology) , cytoplasm , pinus <genus> , botany , transmission electron microscopy , picea abies , fixation (population genetics) , chemistry , biophysics , microbiology and biotechnology , biology , materials science , biochemistry , nanotechnology , gene , enzyme
SUMMARY The method discussed here for preparing conifer needles for transmission electron microscopy combines three important components in a new way: chilling (273 K) during fixation to prevent autolysis, HEPES buffer to protect cytoplasm against washing out and VCD/HXSA as an ultralow viscid and therefore rapidly infiltrating medium. Ultrastructural analysis of Picea abies and Pinus silvestris shows very satisfactory tissue preservation.