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Efficiency of grain‐density autoradiographs with 125 I for the light microscope
Author(s) -
Semmler M.,
Junger E.
Publication year - 1990
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1990.tb03021.x
Subject(s) - emulsion , microscope , materials science , optics , optical microscope , photographic emulsion , radiation , microscopy , chemistry , analytical chemistry (journal) , scanning electron microscope , chromatography , composite material , physics , biochemistry , silver halide
SUMMARY Efficiencies of grain‐density autoradiographs with 125 I for the light microscope were determined using Ilford L4 emulsion and Microdol X development. Calibrated layers of 125 I‐bovine serum albumin and polymer sections of autoradiographic [ 125 I] micro‐scales were used as reference sources. Efficiency was determined as a function of the radiation dose, source thickness and thickness of the absorber foils (e.g. non‐radioactive material between the source and the emulsion), respectively. By describing the dependencies of these factors quantitatively this study provides a means for planning autoradiographic experiments.