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Detection of doubly stained fluorescent specimens using confocal microscopy
Author(s) -
Mossberg Karin,
Ericsson Maria
Publication year - 1990
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1990.tb02995.x
Subject(s) - confocal , fluorescence , fluorescein , excitation wavelength , fluorescein isothiocyanate , confocal microscopy , microscope , lucifer yellow , optical sectioning , microscopy , optics , fluorescence microscope , optical microscope , wavelength , materials science , chemistry , scanning electron microscope , physics , biochemistry , intracellular , gap junction
SUMMARY Studies of doubly stained specimens were performed with a confocal scanning microscope. The instrument used provides the possibility of making separate detections of the fluorescent dyes. The optimal choice of excitation wavelengths and optical filters are discussed. The fluorphores that were used are Lucifer Yellow, Texas Red, fluorescein isothiocyanate and tetramethylrhodamine isothiocyanate.