Premium
Three‐dimensional reconstruction of a plant dictyosome from series of ultrathin sections using computer image processing
Author(s) -
Lockhausen Jürgen,
Kristen Udo,
Menhardt Wido,
Dallas William J.
Publication year - 1990
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1990.tb02993.x
Subject(s) - golgi apparatus , stack (abstract data type) , electron micrographs , endoplasmic reticulum , micrograph , vesicle , perspective (graphical) , image processing , image (mathematics) , computer vision , computer science , electron microscope , artificial intelligence , biology , computer graphics (images) , physics , optics , microbiology and biotechnology , membrane , diffraction , biochemistry , programming language
SUMMARY A single dictyosome from an actively secreting ovary gland cell of Aptenia cordifolia has been reconstructed in 3‐D from a series of twenty‐nine electron micrographs by computer image processing. The reconstruction is presented under different viewing angles in the form of shaded perspective displays. From these displays the entire dictyosome, surrounded by numerous vesicles, appears to be more a spherical than a flat body. The plate‐like region of the dictyósome is demonstrated when only a portion of the electron micrographs is used for the image processing, leading to ‘cut‐off’ displays. Since some upper planes were removed, such ‘cut‐off’ displays revealed both tubular connections between cisternae of the dictyosome and the neighbouring endoplasmic reticulum as well as tubular continuities between adjacent Golgi cisternae within the same stack. Possible consequences of both types of interconnections on transport and processing of proteins and glycoproteins are discussed.