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Three‐dimensional dynamic morphometry on a diffraction pattern in the focal plane
Author(s) -
Ishizaka S.
Publication year - 1990
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1990.tb02990.x
Subject(s) - diffraction , confocal , optics , confocal microscopy , interference (communication) , microsphere , microscopy , materials science , interference microscopy , microscope , phase (matter) , physics , computer science , computer network , channel (broadcasting) , quantum mechanics , chemical engineering , engineering
SUMMARY A new type of morphometry, which shortens the scanning time of precise observation by confocal microscopy, has been investigated. To analyse the 3‐D distribution of active sites on a living cell, microspheres of the same size were immunologically marked on specific sites on a cell. Incident coherent light was scattered on the microspheres and the scattered light from each microsphere superimposed upon each other giving a diffraction pattern of the examined cell. Several series of interference fringes were generated on the diffraction pattern, according to the phase difference of the microspheres. These interference fringes on the 2‐D diffraction pattern enable the (relative) 3‐D positions of the microspheres to be determined. 3‐D dynamic morphometry on a 2‐D diffraction pattern with interference fringes speeds up imaging in confocal microscopy.