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Preparation of the nematode‐trapping fungus, Arthrobotrys oligospora, for scanning electron microscopy by freeze substitution
Author(s) -
Wharton D. A.,
Murray D. S.
Publication year - 1990
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1990.tb02979.x
Subject(s) - cryofixation , fungus , conidium , scanning electron microscope , nematode , liquid nitrogen , biology , botany , chemistry , materials science , ultrastructure , organic chemistry , ecology , composite material
SUMMARY A freeze‐substitution technique for preparing fungal specimens for scanning electron microscopy is described. This involves cryofixation in liquid nitrogen, freeze substitution in methanol at — 20°C and critical‐point drying. The trapping complexes and conidiophores of the nematophagous fungus Arthrobotrys oligospora are well preserved and retain their normal three‐dimensional arrangement.