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Fluorescence histochemical techniques for catecholamines as tools in neurobiology
Author(s) -
Felten David L.,
Felten Suzanne Y.,
Sladek John R.,
Notter Mary D.,
Carlson Sonia L.,
Bellinger Denise L.,
Wiegand Stanley J.
Publication year - 1990
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1990.tb02966.x
Subject(s) - vibratome , immunocytochemistry , biology , glyoxylic acid , immunohistochemistry , fluorescence , central nervous system , paraformaldehyde , immunolabeling , parenchyma , pathology , neuroscience , biochemistry , medicine , physics , botany , quantum mechanics , immunology , endocrinology
SUMMARY Formaldehyde‐induced and glyoxylic‐acid‐induced fluorescence histochemistry permits the tissue localization of catecholamines in the central nervous system (CNS) and peripheral nervous system (PNS), and in culture. Counterstains such as ethidium bromide provide excellent background identification of specific innervated regions in both the CNS and the periphery. Use of fluorescence histochemistry with immunocytochemistry can elucidate catecholamine‐peptide relationships. Gelatin‐ink perfusion used with fluorescence histochemistry permits the investigation of neuro‐vascular relationships and documentation of vascular and parenchymal compartmentation of innervation. Combined use of fluorescence histochemistry and retrograde tracing methods demonstrates the specific cellular sources of innervation of target regions. Micropunch neurochemical analysis provides quantitative data for correlation with fluorescence histochemistry within a target region of innervation, and micro‐spectrofluorometric analysis provides a semi‐quantitative evaluation of the amount of fluorophore within a target region or within specific subcellular compartments such as the cell body or terminals.