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Whole‐mount preparations of mouse lens epithelium for the fluorescent cytological study of actin
Author(s) -
Liou W.
Publication year - 1990
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1990.tb02961.x
Subject(s) - phalloidin , actin , fluorescence , microbiology and biotechnology , cytoskeleton , epithelium , actina , staining , microfilament , biology , fluorescence microscope , actin cytoskeleton , chemistry , biophysics , anatomy , cell , optics , biochemistry , physics , genetics
SUMMARY A technique is given for the preparation of a sheet of epithelial cells from the capsule of the crystalline lens. A new method is described for fixation and staining with fluorescent phalloidin or actin antibody in order to localize the actin cytoskeleton in this tissue. Optical section of the preparation resolves such actin features as apical polygonal arrays, sequestered actin bundles, perinuclear actin aggregates, observed here for the first time, and filamentous networks in the basal region of the cell. This method is superior to previous ones in its ability to preserve actin‐abundant sectors distinctively.