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Methods for obtaining high quality stereoscopic images of microscopic objects
Author(s) -
Sroczyński S. F.
Publication year - 1990
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1990.tb02956.x
Subject(s) - stereoscopy , optics , computer vision , computer science , microscope , observer (physics) , artificial intelligence , image quality , image (mathematics) , physics , quantum mechanics
SUMMARY Over 100 years ago Ernst Abbe developed the so‐called method of half‐moon diaphragms, placed in the exit pupil plane of a binocular microscope, for stereoscopic viewing of microscopic objects. Simplicity of the method being a great advantage, the obvious disadvantages were reduced resolution, azimuthal errors of imaging and failure to control plasticity of the images viewed. Using modern microscopic optics it is possible to avoid these complications, and a complete control of the plasticity of the stereoscopic image as perceived by the observer is achievable while working with medium, strong and the strongest microscope objective lenses. A number of formulae, a nomogram, and a set of tables for calculation of optimal settings are presented in order to promote a range of practical applications, including photomicrography.