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Localization of ribosomal and telomeric DNA sequences in intact plant nuclei by in‐situ hybridization and three‐dimensional optical microscopy
Author(s) -
Rawlins D. J.,
Shaw P. J.
Publication year - 1990
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1990.tb02949.x
Subject(s) - pisum , telomere , in situ , in situ hybridization , sativum , fluorescence in situ hybridization , ribosomal rna , biology , interphase , microscopy , ribosomal dna , complementary dna , dna , microbiology and biotechnology , biophysics , botany , gene , optics , genetics , chemistry , gene expression , chromosome , physics , phylogenetics , organic chemistry
SUMMARY We have combined the use of three‐dimensional (3‐D) fluorescence microscopy and computer image processing of images with in‐situ hybridization to analyse the 3‐D organization of interphase nuclei in plants. In sections of root tips of Pisum sativum , using cDNA probes, we have shown that telomeres are arranged around the nuclear periphery and that the ribosomal genes in this species appear to exist in discrete, 3‐D domains.