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Determination of gold‐labelled surface receptors on single cells by X‐ray microanalysis
Author(s) -
Peschke Th.,
Augsten K.,
Sasama F.,
Gabert A.
Publication year - 1989
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1989.tb02918.x
Subject(s) - receptor , microanalysis , fluorescein isothiocyanate , chemistry , fluorescence , electron probe microanalysis , x ray , analytical chemistry (journal) , scanning electron microscope , biophysics , crystallography , electron microprobe , chromatography , optics , biochemistry , mineralogy , biology , physics , organic chemistry
SUMMARY A standardless X‐ray microanalytical procedure has been developed to determine the number of gold‐labelled surface receptors on whole single cells. The effect of the injection of K 2 PtCl 4 into mice on gold‐labelled concanavalin A (Con A) receptors on peritoneal macrophages was examined with an energy dispersive X‐ray detector in an SEM. The numbers of gold particles seen in electron micrographs and estimated by fluorescence photometric measurements of fluorescein isothiocyanate‐labelled Con A receptors were correlated with the X‐ray microanalytical results.