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Microcomputer system for ion microscopy digital imaging and processing
Author(s) -
Olivo J. C.,
Kahn E.,
Halpern S.,
Briancon C.,
Fragu P.,
DiPaola R.
Publication year - 1989
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1989.tb02909.x
Subject(s) - image processing , microcomputer , computer science , pixel , digital image processing , computer vision , software , artificial intelligence , digital image , computer graphics (images) , microscopy , line (geometry) , image analysis , microscope image processing , computer hardware , image (mathematics) , optics , physics , telecommunications , chip , geometry , mathematics , programming language
SUMMARY Analytical ion microscopy is a powerful tool for biological tissue analysis as it allows direct chemical distribution imaging, even at low element concentrations. A microcomputer based digital imaging system achieving acquisition at low light level is presented. It includes a high sensitivity video camera connected to a specialized image processor subsystem. Acquired images consist of 512 times 512 pixels with 8 bits accuracy. Real‐time image processing software has been implemented so that image processing may be performed on‐line. Image processing software allows off‐line image manipulation and correlation for biological interpretation of elemental mapping images. System capabilities are illustrated by a study of stable and radio iodine mapping in rat thyroid tissue.