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Tomographic reconstruction from energy‐filtered images of thick biological sections
Author(s) -
Olins Ada L.,
Olins Donald E.,
Levy Henri A.,
Margle Stephen M.,
Tinnel Ed P.,
Durfee Richard C.
Publication year - 1989
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1989.tb00588.x
Subject(s) - tilt (camera) , optics , tomographic reconstruction , energy (signal processing) , tomography , physics , materials science , mathematics , geometry , quantum mechanics
SUMMARY Energy filtration makes it possible to image a ∼0.5 μm biological section at 80 kV in the electron microscope. Based on spectra taken at different tilt angles, we chose the most probable energy loss, Δ E p ± 10 eV for each tilt angle, as the imaging energy window. A complete tilt series from + 60° to −60° at 2.5° intervals was collected on the Zeiss EM902 and used in a tomographic reconstruction of transcriptionally active chromatin in the Balbiani ring of Chironomus tentans .