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The effect of aberrations on the axial response of confocal imaging systems
Author(s) -
Wilson T.,
Carlini A. R.
Publication year - 1989
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1989.tb00587.x
Subject(s) - optics , confocal , coma (optics) , detector , lens (geology) , physics , confocal microscopy , microscope , reflector (photography) , materials science , light source
SUMMARY A confocal scanning microscope permits three‐dimensional imaging of a volume specimen. The role of lens aberrations on the axial response of the system is considered both experimentally and theoretically. It is found that primary coma, for example, plays no part in the axial response from a perfect reflector. The role of detector size is also considered and compared with theory. In general, the effect of aberrations is increased as the size of the detector is increased.