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Three‐dimensional imaging in fluorescence by confocal scanning microscopy
Author(s) -
Brakenhoff G. J.,
Voort H. T. M.,
Spronsen E. A.,
Nanninga N.
Publication year - 1989
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1989.tb00555.x
Subject(s) - confocal , confocal microscopy , microscopy , microscope , optical sectioning , materials science , biological specimen , fluorescence , resolution (logic) , fluorescence microscope , fluorescence lifetime imaging microscopy , scanning confocal electron microscopy , confocal laser scanning microscopy , optics , biomedical engineering , computer science , physics , artificial intelligence , medicine
SUMMARY The improved resolution and sectioning capability of a confocal microscope make it an ideal instrument for extracting three‐dimensional information especially from extended biological specimens. The imaging properties, also with finite detection pinholes are considered and a number of biological applications demonstrated.