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A new method for cell culture on an electron‐transparent melamine foil suitable for successive LM, TEM and SEM studies of whole cells
Author(s) -
Westphal Christel,
Hörler Heide,
Pentz Siegwald,
Frösch Dieter
Publication year - 1988
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1988.tb04641.x
Subject(s) - foil method , melamine , transmission electron microscopy , scanning electron microscope , materials science , homogeneous , electron microscope , microscopy , composite material , nanotechnology , optics , mathematics , physics , combinatorics
SUMMARY A new cell culture technique is described which is based on the observation that foils cast from the melamine resin hexamethylol‐melamine‐ether are suitable for the cultivation of beating heart muscle cells and fibroblasts of the rat. This foil can be flamed for sterilization, is about 80 nm in thickness, homogeneous and smooth, withstands dehydration and critical point‐drying, can be removed from glass and permits the imaging of whole cells successively by light microscopy, transmission and scanning electron microscopy. The method is capable of narrowing the gap between light and electron microscopy, yielding excellent whole cell preparations in various kinds of microscopic studies to be performed on one and the same cell.