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Freeze‐fracture of 3T3 cells for high‐resolution scanning electron microscopy
Author(s) -
Haggis G. H.,
Pawley J. B.
Publication year - 1988
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1988.tb04639.x
Subject(s) - scanning electron microscope , materials science , platinum , acceleration voltage , resolution (logic) , electron microscope , coating , microscopy , field electron emission , glutaraldehyde , voltage , high resolution , optics , optoelectronics , nanotechnology , composite material , cathode ray , electron , chemistry , chromatography , electrical engineering , geology , computer science , physics , biochemistry , quantum mechanics , artificial intelligence , catalysis , remote sensing , engineering
SUMMARY Triton‐extracted, freeze‐fractured 3T3 cells have been examined in the Hitachi S‐900 field‐emission SEM, after light platinum coating, at low beam voltage to evaluate the performance of the microscope under these conditions. For unstained material fixed in glutaraldehyde alone, high‐resolution images can be obtained, at accelerating voltages of 1.5‐5kV, after rotary deposition of platinum to an average thickness of 1.5‐3nm. Comparisons are made between these results and those of studies by TEM of deep‐etch replicas of similar material previously published.