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A perfusion chamber for high‐resolution light microscopy of cultured cells *
Author(s) -
Finch Simon A. E.,
Stier Anton
Publication year - 1988
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1988.tb04614.x
Subject(s) - condenser (optics) , microscopy , microscope , materials science , optics , perfusion , resolution (logic) , bright field microscopy , aperture (computer memory) , biomedical engineering , optical microscope , high resolution , physics , computer science , light source , medicine , artificial intelligence , geology , acoustics , scanning electron microscope , remote sensing , cardiology
SUMMARY A perfusion chamber suitable for general microscopic work on upright and inverted microscopes with cultured cells is described. The device features a minimal internal volume and large field of view within small outer dimensions. In particular the chamber body is very flat to accommodate large‐aperture condenser and objective lenses. It is constructed of innocuous, sterilizable materials, incorporates permanent seals for the windows, is robust and simple to use.