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A simple technique for in situ embedding of monolayer cultures in Lowicryl K4M
Author(s) -
BOUGharios G.,
Adams G.,
Moss J.,
Shore I.,
Olscen I.
Publication year - 1988
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1988.tb04606.x
Subject(s) - monolayer , embedding , simple (philosophy) , in situ , materials science , biological system , chemistry , computer science , nanotechnology , biology , artificial intelligence , organic chemistry , philosophy , epistemology
SUMMARY A number of difficulties are encountered in embedding monolayer cultures at low temperature in Lowicryl K4M resin. In this paper we present a simple procedure using Lab‐Tek Flaskettes in which fixation, processing and embedding of monolayer cultures can readily be achieved.