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Contrast in confocal scanning microscopy with a finite detector
Author(s) -
Shuman H.
Publication year - 1988
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1988.tb04562.x
Subject(s) - optics , optical transfer function , microscope , detector , confocal , lens (geology) , contrast (vision) , confocal microscopy , resolution (logic) , optical sectioning , aperture (computer memory) , microscopy , physics , phase contrast imaging , gaussian , contrast transfer function , materials science , phase contrast microscopy , computer science , artificial intelligence , spherical aberration , quantum mechanics , acoustics
SUMMARY The optical properties of a general scanning microscope are determined within the framework of Fourier imaging theory. For a simple model optical system, with Gaussian lens and detector apertures, the contrast transfer function can be expressed in terms of elementary functions. The theory predicts that spatial resolution and depth discrimination vary continuously with detector aperture and that defocus phase contrast is present in transmission images obtained with a symmetric objective, collector lens confocal microscope.