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Application of the GTGO‐AD procedure in the scanning electron microscopy of streptococci adhered to pellicle‐coated dental enamel
Author(s) -
Edwards P. J.,
Jeffryes C. A.
Publication year - 1987
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1987.tb02868.x
Subject(s) - scanning electron microscope , glutaraldehyde , osmium tetroxide , enamel paint , tannic acid , materials science , electron microscope , microscopy , dental enamel , chemistry , environmental scanning electron microscope , dentistry , chromatography , composite material , pathology , medicine , optics , organic chemistry , physics
SUMMARY The scanning electron microscope (SEM) offers a direct and visually effective means of examining streptococci adhering to pellicle‐coated dental enamel. The GTGO‐AD (Glu‐taraldehyde‐Tannic acid‐Guanidine hydrochloride‐Osmium tetroxide‐Air‐Drying procedure) has the advantage that critical point‐drying or freeze‐drying need not be employed. It was found that both the structural and spatial integrity of the adherent streptococci and the pellicle were maintained with the GTGO‐AD procedure.