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A temperature controlled chamber to allow observation and measurement of uptake of fluorochromes into live cells
Author(s) -
Payne J. N.,
Cooper J. D.,
MacKeown S. T.,
Horobin R. W.
Publication year - 1987
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1987.tb02844.x
Subject(s) - phase contrast microscopy , fluorescence , evans blue , fluorescence microscope , microscopy , contrast (vision) , phase (matter) , chemistry , biophysics , materials science , optics , biology , physics , endocrinology , organic chemistry
A temperature controlled cell chamber is described which permits phase‐contrast and fluorescence microscopy of living cells. It is shown how the cellular uptake of fluorochromes can be investigated both photographically and photometrically. Some sample results are presented for the uptake of Evans Blue.