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Computer automated cell size and shape analysis in cryomicroscopy
Author(s) -
Diller Kenneth R.,
Aggarwal Shanti J.
Publication year - 1987
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1987.tb01342.x
Subject(s) - differential interference contrast microscopy , contrast (vision) , phase contrast microscopy , sensitivity (control systems) , calibration , microscopy , microscope , computer science , computer vision , optics , optical microscope , artificial intelligence , phase (matter) , materials science , computer graphics (images) , chemistry , physics , mathematics , statistics , scanning electron microscope , electronic engineering , engineering , organic chemistry
SUMMARY Computer vision techniques have been developed for quantitative analysis of size and shape changes in cells frozen on a cryomicroscope. The analysis is based on implementation of standard serial edge detection algorithms in conjunction with a shape transform to isolate individual cells in complex scenes which may include adjacent and overlying ice crystals. In the present study the sensitivity of the automated analysis procedure is evaluated for images obtained by various microscope optical systems for progressive degrees of subject blurring by defocusing. Size measurements in calibration trials for freezing latex spheres with extracellular ice in the field of view were least sensitive for bright field images, although the most consistent data was obtained by differential interference contrast microscopy. In all cases phase contrast images produced the least accurate data. An example analysis is presented for the freezing of pancreas β‐cells.